In many molecular and diagnostic laboratories, it is necessary to separate certain proteins. One of the most widely used methods for isolating and identifying the target protein from the collection of tissue or blood proteins is the western blot method. With the help of this method, in a relatively short time, the investigated protein can be separated from the collection of cellular proteins, and its concentration can also be roughly estimated. Among the applications of Western Blot technique, it is possible to mention the confirmation of gene expression during an investigated process and the detection of many viral infections.